PucL

From SubtiWiki
Revision as of 11:36, 7 January 2014 by 134.76.70.252 (talk)
Jump to: navigation, search
  • Description: uricase, urate oxidase

Gene name pucL
Synonyms yunL
Essential no
Product uricase
Function purine utilization
Gene expression levels in SubtiExpress: pucL
Metabolic function and regulation of this protein in SubtiPathways:
pucL
MW, pI 56 kDa, 5.484
Gene length, protein length 1482 bp, 494 aa
Immediate neighbours pucK, pucM
Sequences Protein DNA DNA_with_flanks
Genetic context
PucL context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
PucL expression.png















Categories containing this gene/protein

utilization of nucleotides

This gene is a member of the following regulons

PucR regulon, TnrA regulon

The gene

Basic information

  • Locus tag: BSU32450

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO2 (according to Swiss-Prot)
  • Protein family:
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1J2G (with 8-Azaxanthine from Bacillus sp., 66% identity, 79% similarity)
  • KEGG entry: [3]

Additional information

Expression and regulation

  • Regulation:
    • expressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed
    • induced in the presence of purine nucleotides (inducer: allantoin) (PucR) PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References