YqiQ

• Description: methylisocitrate lyase
  
  

Gene name	yqiQ
Synonyms	prpB
Essential	no
Product	methylisocitrate lyase
Function	mother cell metabolism
Gene expression levels in SubtiExpress: yqiQ
Metabolic function and regulation of this protein in SubtiPathways: Coenzyme A, Central C-metabolism
MW, pI	32 kDa, 5.279
Gene length, protein length	903 bp, 301 aa
Immediate neighbours	yqzF, mmgE
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation proteins, membrane proteins, poorly characterized/ putative enzymes

This gene is a member of the following regulons

CcpA regulon, SigE regulon

The gene

Basic information

• Locus tag: BSU24120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate (according to Swiss-Prot)

• Protein family: Methylisocitrate lyase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cell membrane (according to Swiss-Prot)

Database entries

• Structure: 1MUM (enzyme of E. coli), 3KZ2 (the enzyme from B. anthracis)

• UniProt: P54528

• KEGG entry: [3]

• E.C. number: 4.1.3.30

Expression and regulation

• Operon: mmgA-mmgB-mmgC-mmgD-mmgE-yqiQ PubMed

• Expression browser: yqiQ PubMed

• Sigma factor: SigE PubMed

• Regulation:
  • expressed early during sporulation in the mother cell (SigE) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: