MreBH

• Description: cell shape-determining protein, forms filaments, the polymers control/restrict the mobility of the cell wall elongation enzyme complex, , required for LytE activity
  
  

Gene name	mreBH
Synonyms
Essential	no
Product	cell shape-determining protein
Function	cell shape determation
Gene expression levels in SubtiExpress: mreBH
Interactions involving this protein in SubtInteract: MreBH
MW, pI	35 kDa, 5.239
Gene length, protein length	1005 bp, 335 aa
Immediate neighbours	ykpC, abh
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

cell shape, heat shock proteins, membrane proteins

This gene is a member of the following regulons

SigI regulon

The gene

Basic information

• Locus tag: BSU14470

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • forms ring-shaped filaments in a heterologous system PubMed
  • required for the activity of LytE PubMed

• Protein family: ftsA/mreB family (according to Swiss-Prot)

• Paralogous protein(s): MreB, Mbl

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • part of the cell wall biosynthetic complex PubMed
  • MreBH-LytE PubMed
  • MreBH-Mbl PubMed
  • MreB-MreBH PubMed
  • RodZ-MreBH PubMed

• Localization:
  • during logarithmic growth, MreBH forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
  • forms transverse bands as cells enter the stationary phase PubMed
  • close to the inner surface of the cytoplasmic membrane PubMed
  • reports on helical structures formed by MreBH PubMed seem to be misinterpretation of data PubMed
  • normal localization depends on the presence of glucolipids, MreB forms irregular clusters in an ugtP mutant PubMed

Database entries

• Structure:

• UniProt: P39763

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: mreBH PubMed

• Sigma factor: SigI PubMed

• Regulation:
  • induced by heat (SigI) PubMed
  • expression is increased in an ugtP mutant PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage