DprA

• Description: conveys incoming ssDNA to RecA
  
  

Gene name	dprA
Synonyms	smf
Essential	no
Product	recombination mediator protein
Function	DNA repair/ recombination
Gene expression levels in SubtiExpress: dprA
Interactions involving this protein in SubtInteract: DprA
MW, pI	32 kDa, 9.755
Gene length, protein length	891 bp, 297 aa
Immediate neighbours	sucD, topA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU16110

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • provides RecA access to ssDNA during chromosomal transformation (together with RecO) PubMed

• Protein family: smf family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • DprA-RecA PubMed
  • DprA-DprA PubMed
  • DprA-ComFA PubMed
  • SsbB-DprA PubMed

• Localization:

Database entries

• Structure:

• UniProt: P39813

• KEGG entry: [3]

Expression and regulation

• Operon: dprA (according to DBTBS)

• Expression browser: dprA PubMed

• Sigma factor:

• Regulation:
  • expressed under conditions that trigger genetic competence (ComK) PubMed

• Regulatory mechanism:
  • ComK: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Additional publications: PubMed

Tribhuwan Yadav, Begoña Carrasco, Angela R Myers, Nicholas P George, James L Keck, Juan C Alonso
Genetic recombination in Bacillus subtilis: a division of labor between two single-strand DNA-binding proteins.
Nucleic Acids Res: 2012, 40(12);5546-59
[PubMed:22373918] [WorldCat.org] [DOI] (I p)

Serkalem Tadesse, Peter L Graumann
DprA/Smf protein localizes at the DNA uptake machinery in competent Bacillus subtilis cells.
BMC Microbiol: 2007, 7;105
[PubMed:18045469] [WorldCat.org] [DOI] (I e)

Isabelle Mortier-Barrière, Marion Velten, Pauline Dupaigne, Nicolas Mirouze, Olivier Piétrement, Stephen McGovern, Gwennaele Fichant, Bernard Martin, Philippe Noirot, Eric Le Cam, Patrice Polard, Jean-Pierre Claverys
A key presynaptic role in transformation for a widespread bacterial protein: DprA conveys incoming ssDNA to RecA.
Cell: 2007, 130(5);824-36
[PubMed:17803906] [WorldCat.org] [DOI] (P p)

Naomi Kramer, Jeanette Hahn, David Dubnau
Multiple interactions among the competence proteins of Bacillus subtilis.
Mol Microbiol: 2007, 65(2);454-64
[PubMed:17630974] [WorldCat.org] [DOI] (P p)

Janet C Lindow, Masayoshi Kuwano, Shigeki Moriya, Alan D Grossman
Subcellular localization of the Bacillus subtilis structural maintenance of chromosomes (SMC) protein.
Mol Microbiol: 2002, 46(4);997-1009
[PubMed:12421306] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)