Hbs

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  • Description: non-specific DNA-binding protein Hbsu

Gene name hbs
Synonyms dbpA, hupA
Essential yes PubMed
Product non-specific DNA-binding protein Hbsu
Function DNA packaging, function of the signal recognition complex
Gene expression levels in SubtiExpress: hbs
MW, pI 9 kDa, 9.501
Gene length, protein length 276 bp, 92 aa
Immediate neighbours folE, spoIVA
Sequences Protein DNA DNA_with_flanks
Genetic context
Hbs context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
Hbs expression.png















Categories containing this gene/protein

DNA condensation/ segregation, essential genes, membrane proteins, phosphoproteins

This gene is a member of the following regulons

LexA regulon, SigH regulon

The gene

Basic information

  • Locus tag: BSU22790

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • Hbs-mediated untwisting of the DNA stimulates Nth endonuclease activity at AP sites PubMed
  • Protein family: DbpA subfamily (according to Swiss-Prot)
  • Paralogous protein(s): YonN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
    • phosphorylated on Arg-61 PubMed
    • phosphorylation on Thr-4 PubMed
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1HUU (Geobacillus stearothermophilus)
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • induced by DNA damage (LexA) PubMed
    • strongly repressed in response to glucose starvation in M9 medium PubMed
  • Regulatory mechanism:
  • Additional information:
    • mRNA is protected from degradation by RnjA by initiating ribosomes PubMed
    • Hbs is present with about 50,000 copies per genome in vegetative cells and spores, it is one of the most abundant proteins in B. subtilis PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Additional publications: PubMed