PAC5

pAC5: click to enlarge

pAC5 cloning region: click to enlarge

Vector for the construction of translational lacZ fusions that can be integrated at the amyE site

Does not replicate in B. subtilis, integrates into the amyE site

Constructed in the Rapoport lab

• in E. coli: ampicillin resistance

• in B. subtilis: chloramphenicol resistance

• similar vectors: pAC6, pAC7

sequencing primers:

• PAC5R: 5’-CTGCAAGCGATAAGTTGG-3’
• PAC5F: 5’-GCGTAGCGAAAAATCCTTTTC-3‘
• Catcheckrev: 5‘-GTCTGCTTTCTTCATTAGAATCAATCC-3’

I Martin-Verstraete, M Débarbouillé, A Klier, G Rapoport
Mutagenesis of the Bacillus subtilis "-12, -24" promoter of the levanase operon and evidence for the existence of an upstream activating sequence.
J Mol Biol: 1992, 226(1);85-99
[PubMed:1619665] [WorldCat.org] [DOI] (P p)