CysL

• Description: transcriptional activator of the cysJ-cysI operon
  
  

Gene name	cysL
Synonyms	ywfK, ipa-89d
Essential	no
Product	transcriptional regulator (LysR family)
Function	regulation of cysteine biosynthesis
Gene expression levels in SubtiExpress: cysL
Metabolic function and regulation of this protein in SubtiPathways: Cys, Met & Sulfate assimilation
MW, pI	34 kDa, 6.963
Gene length, protein length	897 bp, 299 aa
Immediate neighbours	lipL, pta
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sulfur metabolism, transcription factors and their control

This gene is a member of the following regulons

CysL regulon

The CysL regulon:

• cysJ-cysI, cysL

The gene

Basic information

• Locus tag: BSU37650

Phenotypes of a mutant

• unable to grow with sulfate or sulfite as the only sulfur source PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LysR family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39647

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: cysL PubMed

• Expression browser: cysL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • repressed by sulfate, sulfite, and thiosulfate (CysL) PubMed

• Regulatory mechanism:
  • CysL: auto-repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Isabelle Guillouard, Sandrine Auger, Marie-Françoise Hullo, Farid Chetouani, Antoine Danchin, Isabelle Martin-Verstraete
Identification of Bacillus subtilis CysL, a regulator of the cysJI operon, which encodes sulfite reductase.
J Bacteriol: 2002, 184(17);4681-9
[PubMed:12169591] [WorldCat.org] [DOI] (P p)