MaeA

• Description: malic enzyme
  
  

Gene name	maeA
Synonyms	ywkA
Essential	no
Product	NAD-dependent malate dehydrogenase
Function	malate utilization
Gene expression levels in SubtiExpress: maeA
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	63 kDa, 4.883
Gene length, protein length	1746 bp, 582 aa
Immediate neighbours	ywkB, tdk
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU37050

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-malate + NAD⁺ = pyruvate + CO₂ + NADH (according to Swiss-Prot) malate --> pyruvate

• Protein family: malic enzymes family (according to Swiss-Prot)

• Paralogous protein(s): MalS

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P45868

• KEGG entry: [3]

• E.C. number: 1.1.1.38

Expression and regulation

• Operon: maeA-ywkB PubMed

• Expression browser: maeA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of malate (MalR) PubMed

• Regulatory mechanism:
  • MalR: transcription activation PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant: GP1141 (aphA3) available in Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France