CysE

• Description: serine O-acetyltransferase
  
  

Gene name	cysE
Synonyms	cysA
Essential	no
Product	serine O-acetyltransferase
Function	biosynthesis of cysteine
Gene expression levels in SubtiExpress: cysE
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging, Cys, Met & Sulfate assimilation
MW, pI	23 kDa, 9.489
Gene length, protein length	651 bp, 217 aa
Immediate neighbours	gltX, cysS
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU00930

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine (according to Swiss-Prot)

• Protein family: transferase hexapeptide repeat family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1T3D (from Escherichia coli, 48% identity, 64% similarity) PubMed

• UniProt: Q06750

• KEGG entry: [3]

• E.C. number: 2.3.1.30

Expression and regulation

• Operon: gltX-cysE-cysS-mrnC-yacO-yacP PubMed

• Expression browser: cysE PubMed

• Sigma factor: SigA PubMed

• Regulation: repression by cysteine (T-box) PubMed

• Regulatory mechanism: T-box, overlaps a transcription terminator upstream of cysE: transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Valerie E Pye, Andrew P Tingey, Robert L Robson, Peter C E Moody
The structure and mechanism of serine acetyltransferase from Escherichia coli.
J Biol Chem: 2004, 279(39);40729-36
[PubMed:15231846] [WorldCat.org] [DOI] (P p)

Y Gagnon, R Breton, H Putzer, M Pelchat, M Grunberg-Manago, J Lapointe
Clustering and co-transcription of the Bacillus subtilis genes encoding the aminoacyl-tRNA synthetases specific for glutamate and for cysteine and the first enzyme for cysteine biosynthesis.
J Biol Chem: 1994, 269(10);7473-82
[PubMed:7510287] [WorldCat.org] (P p)