HisA

• Description: phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
  
  

Gene name	hisA
Synonyms
Essential	no
Product	phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase, homologue to HisF
Function	biosynthesis of histidine
Gene expression levels in SubtiExpress: hisA
Metabolic function and regulation of this protein in SubtiPathways: His
MW, pI	26 kDa, 4.842
Gene length, protein length	735 bp, 245 aa
Immediate neighbours	hisF, hisH
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

The gene

Basic information

• Locus tag: BSU34880

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide (according to Swiss-Prot)

• Protein family: hisA/hisF family (according to Swiss-Prot)

• Paralogous protein(s): HisF

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2VEP (from Streptomyces coelicolor, 41% identity, 58% similarity) PubMed

• UniProt: O35006

• KEGG entry: [2]

• E.C. number: 5.3.1.16

Expression and regulation

• Operon: hisZ-hisG-hisD-hisB-hisH-hisA-hisF-hisI PubMed

• Expression browser: hisA PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A626 ( hisA::erm), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)