AspS

• Description: aspartyl-tRNA synthetase
  
  

Gene name	aspS
Synonyms
Essential	yes PubMed
Product	aspartyl-tRNA synthetase
Function	translation
Gene expression levels in SubtiExpress: aspS
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	65 kDa, 4.654
Gene length, protein length	1776 bp, 592 aa
Immediate neighbours	bsrA, hisS
Sequences	Protein DNA Advanced_DNA
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

translation, essential genes, phosphoproteins

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU27550

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1EQR (from Escherichia coli, 52% identity, 68% similarity) PubMed

• UniProt: O32038

• KEGG entry: [2]

• E.C. number: 6.1.1.12

Expression and regulation

• Operon: hisS-aspS

• Expression browser: aspS PubMed

• Sigma factor:

• Regulation:
  • induced by histidine or aspartate limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: