MscL

• Description: large conductance mechanosensitive channel protein, prevents selective release of cytoplasmic proteins in a hypotonic environment
  
  

Gene name	mscL
Synonyms	ywpC
Essential	no
Product	large conductance mechanosensitive channel protein
Function	resistance to osmotic downshockglycine betaine export
Gene expression levels in SubtiExpress: mscL
Metabolic function and regulation of this protein in SubtiPathways: Stress
MW, pI	14 kDa, 4.649
Gene length, protein length	390 bp, 130 aa
Immediate neighbours	ywpD, ywpB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

coping with hypo-osmotic stress, membrane proteins

The gene

Basic information

• Locus tag: BSU36360

Phenotypes of a mutant

sensitive to osmotic downshock (> 0.5 M) during logarithmic growth PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: mscL family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane PubMed

Database entries

• Structure: 3HZQ (MscL of Staphylococcus aureus) PubMed

• UniProt: P94585

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: mscL PubMed

• Expression browser: mscL PubMed

• Sigma factor:

• Regulation: expressed in logarithmic phase PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1A957 ( mscL::spec), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Jan Maarten van Dijl, Groningen, Netherlands

Erhard Bremer, University of Marburg, Germany homepage

References

Reviews

Ian R Booth, Paul Blount
The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.
J Bacteriol: 2012, 194(18);4802-9
[PubMed:22685280] [WorldCat.org] [DOI] (I p)

Ching Kung, Boris Martinac, Sergei Sukharev
Mechanosensitive channels in microbes.
Annu Rev Microbiol: 2010, 64;313-29
[PubMed:20825352] [WorldCat.org] [DOI] (I p)

Valeria Vásquez, Eduardo Perozo
Structural biology: A channel with a twist.
Nature: 2009, 461(7260);47-9
[PubMed:19727188] [WorldCat.org] [DOI] (I p)

Christopher D Pivetti, Ming-Ren Yen, Samantha Miller, Wolfgang Busch, Yi-Hsiung Tseng, Ian R Booth, Milton H Saier
Two families of mechanosensitive channel proteins.
Microbiol Mol Biol Rev: 2003, 67(1);66-85, table of contents
[PubMed:12626684] [WorldCat.org] [DOI] (P p)