GpsA

• Description: glycerol-3-phosphate dehydrogenase (NAD)
  
  

Gene name	gpsA
Synonyms
Essential	yes PubMed
Product	glycerol-3-phosphate dehydrogenase (NAD)
Function	biosynthesis of phospholipids
Gene expression levels in SubtiExpress: gpsA
Metabolic function and regulation of this protein in SubtiPathways: Lipid synthesis
MW, pI	37 kDa, 6.231
Gene length, protein length	1035 bp, 345 aa
Immediate neighbours	yphE, engA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of lipids, essential genes,

The gene

Basic information

• Locus tag: BSU22830

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: sn-glycerol 3-phosphate + NAD(P)⁺ = glycerone phosphate + NAD(P)H (according to Swiss-Prot)

• Protein family: NAD-dependent glycerol-3-phosphate dehydrogenase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:
  • cytoplasm PubMed

Database entries

• Structure: 3K96 (from Coxiella burnetii RSA 493, 37% identity, 59% similarity)

• UniProt: P46919

• KEGG entry: [2]

• E.C. number: 1.1.1.94

Expression and regulation

• Operon:

• Expression browser: gpsA PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ayako Nishibori, Jin Kusaka, Hiroshi Hara, Masato Umeda, Kouji Matsumoto
Phosphatidylethanolamine domains and localization of phospholipid synthases in Bacillus subtilis membranes.
J Bacteriol: 2005, 187(6);2163-74
[PubMed:15743965] [WorldCat.org] [DOI] (P p)

H R Morbidoni, D de Mendoza, J E Cronan
Synthesis of sn-glycerol 3-phosphate, a key precursor of membrane lipids, in Bacillus subtilis.
J Bacteriol: 1995, 177(20);5899-905
[PubMed:7592341] [WorldCat.org] [DOI] (P p)