ProA

• Description: glutamate-5-semialdehyde dehydrogenase, required for normal and osmoadaptive proline biosynthesis
  
  

Gene name	proA
Synonyms
Essential	no
Product	glutamate-5-semialdehyde dehydrogenase
Function	biosynthesis of proline
Gene expression levels in SubtiExpress: proA
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	45 kDa, 5.098
Gene length, protein length	1245 bp, 415 aa
Immediate neighbours	proB, ohrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids, coping with hyper-osmotic stress

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU13130

Phenotypes of a mutant

• auxotrophic for proline PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamate 5-semialdehyde + phosphate + NADP⁺ = L-glutamyl 5-phosphate + NADPH (according to Swiss-Prot)

• Protein family: gamma-glutamyl phosphate reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1O20 (from Thermotoga maritima, 46% identity, 67% similarity) PubMed

• UniProt: P39821

• KEGG entry: [2]

• E.C. number: 1.2.1.41

Expression and regulation

• Operon: proB-proA PubMed

• Expression browser: proA PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:
  • ProA is absent from the membrane proteome of a bdbC-bdbD mutant PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Additional publications: PubMed

Vivianne J Goosens, Ruben A T Mars, Michiel Akeroyd, Andre Vente, Annette Dreisbach, Emma L Denham, Thijs R H M Kouwen, Tjeerd van Rij, Maurien Olsthoorn, Jan Maarten van Dijl
Is proteomics a reliable tool to probe the oxidative folding of bacterial membrane proteins?
Antioxid Redox Signal: 2013, 18(10);1159-64
[PubMed:22540663] [WorldCat.org] [DOI] (I p)

Jeanette Brill, Tamara Hoffmann, Harald Putzer, Erhard Bremer
T-box-mediated control of the anabolic proline biosynthetic genes of Bacillus subtilis.
Microbiology (Reading): 2011, 157(Pt 4);977-987
[PubMed:21233158] [WorldCat.org] [DOI] (I p)

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)