SpoIISB

• Description: disruption blocks sporulation after septum formation
  
  

Gene name	spoIISB
Synonyms
Essential	no
Product	anti-toxin
Function	sporulation
Gene expression levels in SubtiExpress: spoIISB
Interactions involving this protein in SubtInteract: SpoIISB
MW, pI	6 kDa, 10.794
Gene length, protein length	168 bp, 56 aa
Immediate neighbours	xlyA, spoIISA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

sporulation/ other, toxins, antitoxins and immunity against toxins

The gene

Basic information

• Locus tag: BSU12820

Phenotypes of a mutant

• sporulation efficiency is reduced by four orders of magnitude PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SpoIISA-SpoIISB PubMed

• Localization:

Database entries

• Structure: 3O6Q (cytoplasmic fragment of SpoIISA (CSpoIISA) in complex with SpoIISB) PubMed

• UniProt: O34800

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:
• spoIISA-spoIISB PubMed
• spoIISB PubMed

• Expression browser: spoIISB PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • 1S124 ( spoIISB::tet), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Patrik Florek, Vladimir M Levdikov, Elena Blagova, Andrey A Lebedev, Rostislav Škrabana, Stanislava Resetárová, Pamela Pavelcíková, Imrich Barak, Anthony J Wilkinson
The structure and interactions of SpoIISA and SpoIISB, a toxin-antitoxin system in Bacillus subtilis.
J Biol Chem: 2011, 286(8);6808-19
[PubMed:21147767] [WorldCat.org] [DOI] (I p)

Patrik Florek, Katarína Muchová, Pamela Pavelcíková, Imrich Barák
Expression of functional Bacillus SpoIISAB toxin-antitoxin modules in Escherichia coli.
FEMS Microbiol Lett: 2008, 278(2);177-84
[PubMed:18096016] [WorldCat.org] [DOI] (P p)

E Adler, I Barák, P Stragier
Bacillus subtilis locus encoding a killer protein and its antidote.
J Bacteriol: 2001, 183(12);3574-81
[PubMed:11371520] [WorldCat.org] [DOI] (P p)