SbcC

• Description: DNA exonuclease
  
  

Gene name	sbcC
Synonyms	yirY
Essential	no
Product	DNA exonuclease
Function	DNA inter-strand cross-link repair
Gene expression levels in SubtiExpress: sbcC
Interactions involving this protein in SubtInteract: SbcC
MW, pI	128 kDa, 5.294
Gene length, protein length	3390 bp, 1130 aa
Immediate neighbours	sbcD, yisB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

DNA repair/ recombination, genetic competence

This gene is a member of the following regulons

ComK regulon

The gene

Basic information

• Locus tag: BSU10650

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: SbcC subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:
  • SsbA-SbcC PubMed

• Localization: Nucleoid (Mid-cell) PubMed

Database entries

• Structure:

• UniProt: O06714

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sbcD-sbcC-yisB (according to DBTBS)

• Expression browser: sbcC PubMed

• Sigma factor:

• Regulation:
  • positive regulation by ComK PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:
  • GP894 (sbcCD::kan), available in Stülke lab
  • 1A898 ( sbcC::cat), PubMed, available at BGSC

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Peter Graumann, Freiburg University, Germany homepage

References

Original publications

Judita Mascarenhas, Humberto Sanchez, Serkalem Tadesse, Dawit Kidane, Mahalakshmi Krisnamurthy, Juan C Alonso, Peter L Graumann
Bacillus subtilis SbcC protein plays an important role in DNA inter-strand cross-link repair.
BMC Mol Biol: 2006, 7;20
[PubMed:16780573] [WorldCat.org] [DOI] (I e)

Jean-Christophe Meile, Ling Juan Wu, S Dusko Ehrlich, Jeff Errington, Philippe Noirot
Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory.
Proteomics: 2006, 6(7);2135-46
[PubMed:16479537] [WorldCat.org] [DOI] (P p)

Mitsuo Ogura, Hirotake Yamaguchi, Kazuo Kobayashi, Naotake Ogasawara, Yasutaro Fujita, Teruo Tanaka
Whole-genome analysis of genes regulated by the Bacillus subtilis competence transcription factor ComK.
J Bacteriol: 2002, 184(9);2344-51
[PubMed:11948146] [WorldCat.org] [DOI] (P p)

Additional publications: PubMed