LacR

• Description: transcriptional repressor of the beta-galactosidase gene
  
  

Gene name	lacR
Synonyms	yvfJ
Essential	no
Product	transcriptional repressor (LacI family)
Function	regulation of utilization of polymeric carbohydrates
MW, pI	37 kDa, 5.396
Gene length, protein length	990 bp, 330 aa
Immediate neighbours	cycB, lutR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, transcription factors and their control

The LacR regulon:

• cycB-yvfL-yvfM-lacA-yvfO

The gene

Basic information

• Locus tag: BSU34170

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: LacI family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: O07008

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: lacR PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant: GP815 (spc) available in the Stülke lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

R A Daniel, J Haiech, F Denizot, J Errington
Isolation and characterization of the lacA gene encoding beta-galactosidase in Bacillus subtilis and a regulator gene, lacR.
J Bacteriol: 1997, 179(17);5636-8
[PubMed:9287030] [WorldCat.org] [DOI] (P p)

J Errington, C H Vogt
Isolation and characterization of mutations in the gene encoding an endogenous Bacillus subtilis beta-galactosidase and its regulator.
J Bacteriol: 1990, 172(1);488-90
[PubMed:2104611] [WorldCat.org] [DOI] (P p)