YwkB

• Description: unknown
  
  

Gene name	ywkB
Synonyms
Essential	no
Product	unknown
Function	unknown
Metabolic function and regulation of this protein in SubtiPathways: Central C-metabolism
MW, pI	33 kDa, 7.108
Gene length, protein length	957 bp, 319 aa
Immediate neighbours	racA, maeA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

membrane proteins, proteins of unknown function

This gene is a member of the following regulons

MalR regulon

The gene

Basic information

• Locus tag: BSU37040

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: P45869

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: maeA-ywkB PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed in the presence of malate (MalR) PubMed

• Regulatory mechanism:
  • MalR: transcription activation PubMed

• Additional information:
  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lehnik-Habrink M, Schaffer M, Mäder U, Diethmaier C, Herzberg C, Stülke J  
RNA processing in Bacillus subtilis: identification of targets of the essential RNase Y. 
Mol Microbiol. 2011 81(6): 1459-1473. 
PubMed:21815947

Thierry Doan, Pascale Servant, Shigeo Tojo, Hirotake Yamaguchi, Guillaume Lerondel, Ken-Ichi Yoshida, Yasutaro Fujita, Stéphane Aymerich
The Bacillus subtilis ywkA gene encodes a malic enzyme and its transcription is activated by the YufL/YufM two-component system in response to malate.
Microbiology (Reading): 2003, 149(Pt 9);2331-2343
[PubMed:12949160] [WorldCat.org] [DOI] (P p)