Nap

• Description: carboxylesterase NA
  
  

Gene name	nap
Synonyms	cesA
Essential	no
Product	carboxylesterase NA
Function	lipid degradation
MW, pI	33 kDa, 5.798
Gene length, protein length	900 bp, 300 aa
Immediate neighbours	ydfJ, ydfK
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

utilization of lipids

The gene

Basic information

• Locus tag: BSU05440

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: A carboxylic ester + H₂O = an alcohol + a carboxylate (according to Swiss-Prot)

• Protein family: AB hydrolase superfamily (according to Swiss-Prot)

• Paralogous protein(s): YbfK

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2R11

• UniProt: P96688

• KEGG entry: [3]

• E.C. number: 3.1.1.1 3.1.1.1]

Expression and regulation

• Operon: nap PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Luis F Godinho, Carlos R Reis, Henriëtte J Rozeboom, Frank J Dekker, Bauke W Dijkstra, Gerrit J Poelarends, Wim J Quax
Enhancement of the enantioselectivity of carboxylesterase A by structure-based mutagenesis.
J Biotechnol: 2012, 158(1-2);36-43
[PubMed:22248594] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)