ResB

• Description: part of haem translocase, required for cytochrome c synthesis
  
  

Gene name	resB
Synonyms	ypxB
Essential	yes PubMed
Product	part of the ResB-ResC haem translocase
Function	cytochrome c biogenesis
Interactions involving this protein in SubtInteract: ResB
MW, pI	61 kDa, 9.474
Gene length, protein length	1626 bp, 542 aa
Immediate neighbours	resC, resA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

respiration, essential genes, membrane proteins

This gene is a member of the following regulons

CcpA regulon, PhoP regulon, ResD regulon

The gene

Basic information

• Locus tag: BSU23140

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: haem-binding protein, translocation of haem from the cytoplasmic to the extracytoplasmic site of the membrane PubMed

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: ResB-ResC PubMed

• Localization: cell membrane ResB-ResC PubMed

Database entries

• Structure:

• UniProt: P35161

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: resA-resB-resC-resD-resE PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • expressed under anaerobic conditions (ResD) PubMed
  • expressed under conditions of phosphate limitation (PhoP) PubMed
  • repressed by glucose (CcpA) PubMed

• Regulatory mechanism:
  • ResD: transcription activation PubMed
  • PhoP: transcription activation PubMed
  • CcpA: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Umesh Ahuja, Peter Kjelgaard, Benjamin L Schulz, Linda Thöny-Meyer, Lars Hederstedt
Haem-delivery proteins in cytochrome c maturation System II.
Mol Microbiol: 2009, 73(6);1058-71
[PubMed:19682263] [WorldCat.org] [DOI] (I p)

Soo-Keun Choi, Milton H Saier
Mechanism of CcpA-mediated glucose repression of the resABCDE operon of Bacillus subtilis.
J Mol Microbiol Biotechnol: 2006, 11(1-2);104-10
[PubMed:16825793] [WorldCat.org] [DOI] (P p)

M M Nakano, Y Zhu
Involvement of ResE phosphatase activity in down-regulation of ResD-controlled genes in Bacillus subtilis during aerobic growth.
J Bacteriol: 2001, 183(6);1938-44
[PubMed:11222591] [WorldCat.org] [DOI] (P p)

N E Le Brun, J Bengtsson, L Hederstedt
Genes required for cytochrome c synthesis in Bacillus subtilis.
Mol Microbiol: 2000, 36(3);638-50
[PubMed:10844653] [WorldCat.org] [DOI] (P p)

S M Birkey, W Liu, X Zhang, M F Duggan, F M Hulett
Pho signal transduction network reveals direct transcriptional regulation of one two-component system by another two-component regulator: Bacillus subtilis PhoP directly regulates production of ResD.
Mol Microbiol: 1998, 30(5);943-53
[PubMed:9988472] [WorldCat.org] [DOI] (P p)

G Sun, E Sharkova, R Chesnut, S Birkey, M F Duggan, A Sorokin, P Pujic, S D Ehrlich, F M Hulett
Regulators of aerobic and anaerobic respiration in Bacillus subtilis.
J Bacteriol: 1996, 178(5);1374-85
[PubMed:8631715] [WorldCat.org] [DOI] (P p)