HemL

• Description: glutamate-1-semialdehyde aminotransferase
  
  

Gene name	hemL
Synonyms	hemK
Essential	no
Product	glutamate-1-semialdehyde aminotransferase
Function	heme biosynthesis
MW, pI	46 kDa, 5.055
Gene length, protein length	1290 bp, 430 aa
Immediate neighbours	spoVID, hemB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis of cofactors

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU28120

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: (S)-4-amino-5-oxopentanoate = 5-aminolevulinate (according to Swiss-Prot)

• Protein family: HemL subfamily (according to Swiss-Prot)

• Paralogous protein(s): GsaB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 3BS8 PubMed

• UniProt: P30949

• KEGG entry: [3]

• E.C. number: 5.4.3.8

Expression and regulation

• Operon: hemA-hemX-hemC-hemD-hemB-hemL PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • induced by hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Honghua Ge, Xinhuai Lv, Jun Fan, Yongxiang Gao, Maikun Teng, Liwen Niu
Crystal structure of glutamate-1-semialdehyde aminotransferase from Bacillus subtilis with bound pyridoxamine-5'-phosphate.
Biochem Biophys Res Commun: 2010, 402(2);356-60
[PubMed:20946885] [WorldCat.org] [DOI] (I p)

A F Herbig, J D Helmann
Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA.
Mol Microbiol: 2001, 41(4);849-59
[PubMed:11532148] [WorldCat.org] [DOI] (P p)

Per Johansson, Lars Hederstedt
Organization of genes for tetrapyrrole biosynthesis in gram--positive bacteria.
Microbiology (Reading): 1999, 145 ( Pt 3);529-538
[PubMed:10217486] [WorldCat.org] [DOI] (P p)

M Hansson, L Rutberg, I Schröder, L Hederstedt
The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III.
J Bacteriol: 1991, 173(8);2590-9
[PubMed:1672867] [WorldCat.org] [DOI] (P p)