PGP888

pGP888: click to enlarge

The vector was constructed in the Stülke lab and it is suitable for the xylose-inducible expression of proteins in B. subtilis. The plasmid confers resistance to ampicillin and kanamycin in E. coli and B. subtilis, respectively. To transform B. subtilis, the plasmid has to be linearized with ScaI or NotI

Hints for usage

• An additional base has to be inserted in the forward primer to restore the frame.
• Don't forget a stop codon in the reverse primer.

The reference

Diethmaier C, Pietack N, Gunka K, Wrede C, Lehnik-Habrink M, Herzberg C, Hübner S, Stülke J    
A Novel Factor Controlling Bistability in Bacillus subtilis: The YmdB Protein Affects
Flagellin Expression and Biofilm Formation. 
J Bacteriol. 2011 Aug 19. [Epub ahead of print]
PubMed:21856853