PfeT

• Description: zinc transporter
  
  

Gene name	zosA
Synonyms	ykvW
Essential	no
Product	P-type zinc-transporting ATPase
Function	zinc uptake
MW, pI	68 kDa, 4.934
Gene length, protein length	1911 bp, 637 aa
Immediate neighbours	stoA, ykvY
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU13850

Phenotypes of a mutant

• • reduced genetic competence, can be rescued by the addition of excess zinc PubMed
  • reduced expression of the comK operon, control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Zn²⁺(In) = ADP + phosphate + Zn²⁺(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase PubMed

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• UniProt: O31688

• KEGG entry: [2]

• E.C. number: 3.6.3.5

Expression and regulation

• Operon: zosA PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by hydrogen peroxide (PerR) PubMed
  • repressed in the absence of hydrogen peroxide (PerR) PubMed

• Regulatory mechanism: PerR: transcription repression PubMed
  • PerR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between stoA and zosA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis.
Mol Microbiol: 2002, 45(4);997-1005
[PubMed:12180919] [WorldCat.org] [DOI] (P p)