MreC
- Description: MreC is a cell shape determining protein, it couples the cytosolic MreB and MreB-like proteins to the extracellular peptidoglycan-synthesizing machinery
| Gene name | mreC |
| Synonyms | |
| Essential | yes PubMed |
| Product | cell shape-determining protein |
| Function | cell shape determation |
| MW, pI | 32 kDa, 6.248 |
| Gene length, protein length | 870 bp, 290 aa |
| Immediate neighbours | mreD, mreB |
| Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context 
This image was kindly provided by SubtiList
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Contents
Categories containing this gene/protein
cell shape, cell envelope stress proteins (controlled by SigM, W, X, Y), essential genes, membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU28020
Phenotypes of a mutant
- mreC is essential under normal conditions PubMed.
- Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for mreC in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like rodA and the redundant gene pair pbpA and pbpH.
- Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated PubMed.
- mreC can be deleted provided that 0.5 M sucrose and 20 mM Mg(2+) is provided in the media, mreC is therefore conditionally essentail. The phenotype of the mreC deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters PubMed.
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
Function
MreC functions in cell wall synthesis by, together with the MreB cytoskeleton, localizing the cell wall synthetic machinery to the correct part of the cell. MreC therefore ensures that the cell wall is made in the correct way to maintain the proper shape of the cell.
MreC in other organisms
MreC has been studied in other organisms where it has been shown to be important in cell shape determination.
- Escherichia coli PubMed PubMed
- Caulobacter cresentus PubMed PubMed
- Rhodobacter spheroides PubMed
- Streptomyces coelicolor PubMed
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: None/ structural protein
- Protein family: mreC family (according to Swiss-Prot) COG1793
- Paralogous protein(s): None
Extended information on the protein
- Kinetic information:
- Domains: Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.
- Modification:
- Cofactor(s):
- Localization:
- trans-membrane protein PubMed
- during logarithmic growth, MreD forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis PubMed
- forms transverse bands as cells enter the stationary phase PubMed
- reports on helical structures formed by MreC PubMed seem to be misinterpretation of data PubMed
Database entries
- Structure:
- UniProt: Q01466
- KEGG entry: [3]
- E.C. number:
Additional information
Expression and regulation
- Regulation:
- Regulatory mechanism:
- Additional information:
Biological materials
- Mutant: A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the Errington lab PubMed.
- Expression vector:
- lacZ fusion:
- GFP fusion: A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 PubMed.
- two-hybrid system:
- Antibody: antisera raised in rabit is avaliable from the Errington lab.
Labs working on this gene/protein
Jeff Errington, Newcastle University, UK homepage
Peter Graumann, Freiburg University, Germany homepage
Your additional remarks
mreC is an abbreviation of murein region e, gene C
References
Localization
Other original publications
