QdoI

• Description: Fe-containing quercetin 2,3-dioxygenase
  
  

Gene name	qdoI
Synonyms	yxaG
Essential	no
Product	Fe-containing quercetin 2,3-dioxygenase
Function	resistance to plant product quercetin
MW, pI	37,4 kDa, 5.562
Gene length, protein length	1011 bp, 337 aa
Immediate neighbours	yxaH, yxaF
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

This gene is a member of the following regulons

LmrA regulon, YxaF regulon

The gene

Basic information

• Locus tag: BSU39980

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Quercetin + O₂ = 2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate + CO + H⁺ (according to Swiss-Prot)

• Protein family:bicupin family

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s): Fe(2+)

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 2H0V

• UniProt: P42106

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: qdoI-yxaH PubMed

• Sigma factor:

• Regulation:
  • induced by flavonoids such as quercetin (YxaF, LmrA) PubMed

• Regulatory mechanism:
  • LmrA: transcription repression PubMed
  • YxaF: transcription repression PubMed

• Additional information:
  • major regulator: YxaF PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Kazutake Hirooka, Yasutaro Fujita
Excess production of Bacillus subtilis quercetin 2,3-dioxygenase affects cell viability in the presence of quercetin.
Biosci Biotechnol Biochem: 2010, 74(5);1030-8
[PubMed:20460727] [WorldCat.org] [DOI] (I p)

Kazutake Hirooka, Satoshi Kunikane, Hiroshi Matsuoka, Ken-Ichi Yoshida, Kanako Kumamoto, Shigeo Tojo, Yasutaro Fujita
Dual regulation of the Bacillus subtilis regulon comprising the lmrAB and yxaGH operons and yxaF gene by two transcriptional repressors, LmrA and YxaF, in response to flavonoids.
J Bacteriol: 2007, 189(14);5170-82
[PubMed:17483215] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Yo-Hei Ohki, Makiko Murata, Masaki Kinehara, Hiroshi Matsuoka, Takenori Satomura, Reiko Ohki, Miyuki Kumano, Kunio Yamane, Yasutaro Fujita
Bacillus subtilis LmrA is a repressor of the lmrAB and yxaGH operons: identification of its binding site and functional analysis of lmrB and yxaGH.
J Bacteriol: 2004, 186(17);5640-8
[PubMed:15317768] [WorldCat.org] [DOI] (P p)

Laura Bowater, Shirley A Fairhurst, Victoria J Just, Stephen Bornemann
Bacillus subtilis YxaG is a novel Fe-containing quercetin 2,3-dioxygenase.
FEBS Lett: 2004, 557(1-3);45-8
[PubMed:14741339] [WorldCat.org] [DOI] (P p)

Jim M Dunwell, Alan Purvis, Sawsan Khuri
Cupins: the most functionally diverse protein superfamily?
Phytochemistry: 2004, 65(1);7-17
[PubMed:14697267] [WorldCat.org] [DOI] (P p)

Ken-Ichi Yoshida, Izumi Ishio, Eishi Nagakawa, Yoshiyuki Yamamoto, Mami Yamamoto, Yasutaro Fujita
Systematic study of gene expression and transcription organization in the gntZ-ywaA region of the Bacillus subtilis genome.
Microbiology (Reading): 2000, 146 ( Pt 3);573-579
[PubMed:10746760] [WorldCat.org] [DOI] (P p)