CimH

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  • Description: transporter for citrate and malate (proton symport)

Gene name cimH
Synonyms yxkJ
Essential no
Product transporter for citrate and malate (proton symport)
Function citrate and malate uptake
Metabolic function and regulation of this protein in SubtiPathways:
Central C-metabolism
MW, pI 48 kDa, 6.554
Gene length, protein length 1350 bp, 450 aa
Immediate neighbours cydA, yxkI
Get the DNA and protein sequences
(Barbe et al., 2009)
Genetic context
YxkJ context.gif
This image was kindly provided by SubtiList







Categories containing this gene/protein

transporters/ other, utilization of specific carbon sources, membrane proteins

This gene is a member of the following regulons

CcpA regulon

The gene

Basic information

  • Locus tag: BSU38770

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: uptake of citrate and malate (symport with protons) PubMed
  • Protein family: sodium:citrate (SCF) symporter family (according to Swiss-Prot) 2-hydroxycarboxylate transporter family
  • Paralogous protein(s): MaeN

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:
  • Interactions:
  • Localization: cell membrane (according to Swiss-Prot)

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation: expression profile

  • Operon:
  • Regulatory mechanism:
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References

Reviews

Original publications

Bastiaan P Krom, Juke S Lolkema
Conserved residues R420 and Q428 in a cytoplasmic loop of the citrate/malate transporter CimH of Bacillus subtilis are accessible from the external face of the membrane.
Biochemistry: 2003, 42(2);467-74
[PubMed:12525174] [WorldCat.org] [DOI] (P p)

B P Krom, R Aardema, J S Lolkema
Bacillus subtilis YxkJ is a secondary transporter of the 2-hydroxycarboxylate transporter family that transports L-malate and citrate.
J Bacteriol: 2001, 183(20);5862-9
[PubMed:11566984] [WorldCat.org] [DOI] (P p)

Y Miwa, A Nakata, A Ogiwara, M Yamamoto, Y Fujita
Evaluation and characterization of catabolite-responsive elements (cre) of Bacillus subtilis.
Nucleic Acids Res: 2000, 28(5);1206-10
[PubMed:10666464] [WorldCat.org] [DOI] (I p)