ProA

• Description: glutamate-5-semialdehyde dehydrogenase
  
  

Gene name	proA
Synonyms
Essential	no
Product	glutamate-5-semialdehyde dehydrogenase
Function	biosynthesis of proline
Metabolic function and regulation of this protein in SubtiPathways: Pro, Stress
MW, pI	45 kDa, 5.098
Gene length, protein length	1245 bp, 415 aa
Immediate neighbours	proB, ohrA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

T-box

The gene

Basic information

• Locus tag: BSU13130

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: L-glutamate 5-semialdehyde + phosphate + NADP⁺ = L-glutamyl 5-phosphate + NADPH (according to Swiss-Prot)

• Protein family: gamma-glutamyl phosphate reductase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 1O20 (from Thermotoga maritima, 46% identity, 67% similarity) PubMed

• UniProt: P39821

• KEGG entry: [2]

• E.C. number: 1.2.1.41

Expression and regulation

• Operon: proB-proA

• Sigma factor:

• Regulation:
  • induced by proline limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, transcriptional antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Mingqing Chen, Hongbo Wei, JunWei Cao, Ruijie Liu, Youliang Wang, Congyi Zheng
Expression of Bacillus subtilis proBA genes and reduction of feedback inhibition of proline synthesis increases proline production and confers osmotolerance in transgenic Arabidopsis.
J Biochem Mol Biol: 2007, 40(3);396-403
[PubMed:17562291] [WorldCat.org] [DOI] (P p)