LeuS

• Description: Leu-tRNA synthetase
  
  

Gene name	leuS
Synonyms
Essential	yes PubMed
Product	leucyl-tRNA synthetase
Function	translation
Metabolic function and regulation of this protein in SubtiPathways: tRNA charging
MW, pI	91 kDa, 4.857
Gene length, protein length	2412 bp, 804 aa
Immediate neighbours	ytwF, ytvB
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU30320

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

translation, essential genes

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu) (according to Swiss-Prot)

• Protein family: class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2AJG (from Escherichia coli, 39% identity, 56% similarity) PubMed

• UniProt: P36430

• KEGG entry: [3]

• E.C. number: 6.1.1.4

Expression and regulation

• Operon: leuS PubMed

• Sigma factor:

• Regulation:
  • induced by leucine limitation (T-box) PubMed

• Regulatory mechanism:
  • T-box: RNA switch, binding of uncharged tRNA-Leu results in transcription antitermination PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Reviews

Ana Gutiérrez-Preciado, Tina M Henkin, Frank J Grundy, Charles Yanofsky, Enrique Merino
Biochemical features and functional implications of the RNA-based T-box regulatory mechanism.
Microbiol Mol Biol Rev: 2009, 73(1);36-61
[PubMed:19258532] [WorldCat.org] [DOI] (I p)

Original Publications

P B Vander Horn, S A Zahler
Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis.
J Bacteriol: 1992, 174(12);3928-35
[PubMed:1317842] [WorldCat.org] [DOI] (P p)