Hbs

• Description: non-specific DNA-binding protein Hbsu
  
  

Gene name	hbs
Synonyms	dbpA
Essential	yes PubMed
Product	non-specific DNA-binding protein Hbsu
Function	DNA packaging, function of the signal recognition complex
MW, pI	9 kDa, 9.501
Gene length, protein length	276 bp, 92 aa
Immediate neighbours	folE, spoIVA
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22790

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

DNA condensation/ segregation, essential genes, membrane proteins, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: DbpA subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Thr-4 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot), membrane associated PubMed

Database entries

• Structure: 1HUU (Geobacillus stearothermophilus), 1EXE (phage SPO1 transcription factor, 45% identity)

• UniProt: P08821

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hbs PubMed

• Sigma factor: SigA PubMed, SigH PubMed

• Regulation:
  • induced by DNA damage (LexA) PubMed

• Regulatory mechanism:
  • LexA: transcription repression PubMed

• Additional information: mRNA is protected from degradation by RnjA by initiating ribosomes PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Ciaran Condon, IBPC, Paris, France Homepage

References

Reviews

Wolfgang Klein, Mohamed A Marahiel
Structure-function relationship and regulation of two Bacillus subtilis DNA-binding proteins, HBsu and AbrB.
J Mol Microbiol Biotechnol: 2002, 4(3);323-9
[PubMed:11931565] [WorldCat.org] (P p)