Ndk

• Description: nucleoside diphosphate kinase
  
  

Gene name	ndk
Synonyms
Essential	no
Product	nucleoside diphosphate kinase
Function	maintainance of an equilibrium between the concentrations of different nucleoside triphosphates
Metabolic function and regulation of this protein in SubtiPathways: Pyrimidines, Purine salvage
MW, pI	16 kDa, 5.568
Gene length, protein length	447 bp, 149 aa
Immediate neighbours	cheR, hepT
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22730

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

nucleotide metabolism/ other, phosphoproteins

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + nucleoside diphosphate = ADP + nucleoside triphosphate (according to Swiss-Prot)

• Protein family: phosphate acetyltransferase and butyryltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Thr-92 AND Ser-123 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure: 1WKJ (from Thermus thermophilus, 59% identity, 82% similarity)

• UniProt: P31103

• KEGG entry: [2]

• E.C. number: 2.7.4.6

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)