DefA

• Description: formylmethionine deformylase
  
  

Gene name	def
Synonyms	yloK
Essential	no
Product	formylmethionine deformylase
Function	translation
MW, pI	17 kDa, 4.731
Gene length, protein length	480 bp, 160 aa
Immediate neighbours	priA, fmt
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU15720

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

Categories containing this gene/protein

translation, protein modification

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Formyl-L-methionyl peptide + H₂O = formate + methionyl peptide (according to Swiss-Prot)

• Protein family: polypeptide deformylase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P94462

• KEGG entry: [2]

• E.C. number: 3.5.1.88

Expression and regulation

• Operon: def-fmt-yloM PubMed

• Sigma factor: SigA PubMed

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Krzysztof Hinc, Adam Iwanicki, Simone Seror, Michał Obuchowski
Mapping of a transcription promoter located inside the priA gene of the Bacillus subtilis chromosome.
Acta Biochim Pol: 2006, 53(3);497-505
[PubMed:16964327] [WorldCat.org] (P p)

Julia Elisabeth Bandow, Dörte Becher, Knut Büttner, Falko Hochgräfe, Christoph Freiberg, Heike Brötz, Michael Hecker
The role of peptide deformylase in protein biosynthesis: a proteomic study.
Proteomics: 2003, 3(3);299-306
[PubMed:12627383] [WorldCat.org] [DOI] (P p)

Michael Haas, Dieter Beyer, Reinhold Gahlmann, Christoph Freiberg
YkrB is the main peptide deformylase in Bacillus subtilis, a eubacterium containing two functional peptide deformylases.
Microbiology (Reading): 2001, 147(Pt 7);1783-1791
[PubMed:11429456] [WorldCat.org] [DOI] (P p)