HemY

• Description: protoporphyrinogen IX oxidase
  
  

Gene name	hemY
Synonyms	hemG
Essential	no
Product	protoporphyrinogen IX oxidase
Function	heme biosynthesis
MW, pI	51 kDa, 8.042
Gene length, protein length	1410 bp, 470 aa
Immediate neighbours	hemH, yhgD
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU10140

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

Categories containing this gene/protein

biosynthesis of cofactors

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: Protoporphyrinogen-IX + 3 O₂ = protoporphyrin-IX + 3 H₂O₂ (according to Swiss-Prot)

• Protein family: protoporphyrinogen oxidase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 3I6D

• UniProt: P32397

• KEGG entry: [3]

• E.C. number: 1.3.3.4

Expression and regulation

• Operon: hemE-hemH-hemY PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Xiaohong Qin, Lu Sun, Xin Wen, Xue Yang, Ying Tan, Hao Jin, Qiongyao Cao, Weihong Zhou, Zhen Xi, Yuequan Shen
Structural insight into unique properties of protoporphyrinogen oxidase from Bacillus subtilis.
J Struct Biol: 2010, 170(1);76-82
[PubMed:19944166] [WorldCat.org] [DOI] (I p)

Lu Sun, Xin Wen, Ying Tan, Heyang Li, Xing Yang, Yuefang Zhao, Baifan Wang, Qiongyao Cao, Congwei Niu, Zhen Xi
Site-directed mutagenesis and computational study of the Y366 active site in Bacillus subtilis protoporphyrinogen oxidase.
Amino Acids: 2009, 37(3);523-30
[PubMed:19266155] [WorldCat.org] [DOI] (I p)

M Hansson, M C Gustafsson, C G Kannangara, L Hederstedt
Isolated Bacillus subtilis HemY has coproporphyrinogen III to coproporphyrin III oxidase activity.
Biochim Biophys Acta: 1997, 1340(1);97-104
[PubMed:9217019] [WorldCat.org] [DOI] (P p)

M Hansson, L Hederstedt
Bacillus subtilis HemY is a peripheral membrane protein essential for protoheme IX synthesis which can oxidize coproporphyrinogen III and protoporphyrinogen IX.
J Bacteriol: 1994, 176(19);5962-70
[PubMed:7928957] [WorldCat.org] [DOI] (P p)

M Hansson, L Hederstedt
Cloning and characterization of the Bacillus subtilis hemEHY gene cluster, which encodes protoheme IX biosynthetic enzymes.
J Bacteriol: 1992, 174(24);8081-93
[PubMed:1459957] [WorldCat.org] [DOI] (P p)