YckE

• Description: aryl-β-glucosidase
  
  

Gene name	yckE
Synonyms	bglC
Essential	no
Product	aryl-β-glucosidase
Function	utilization of aryl-β-glucosides
MW, pI	54 kDa, 5.575
Gene length, protein length	1431 bp, 477 aa
Immediate neighbours	yckD, nin
Gene sequence (+200bp)	Protein sequence
Caution: The sequence for this gene in SubtiList contains errors
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU03410

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 1 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P42403

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: yckE (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Lun-Cheng Kuo, Kung-Ta Lee
Cloning, expression, and characterization of two beta-glucosidases from isoflavone glycoside-hydrolyzing Bacillus subtilis natto.
J Agric Food Chem: 2008, 56(1);119-25
[PubMed:18069788] [WorldCat.org] [DOI] (P p)

B Setlow, R-M Cabrera-Martinez, P Setlow
Mechanism of the hydrolysis of 4-methylumbelliferyl-beta-D-glucoside by germinating and outgrowing spores of Bacillus species.
J Appl Microbiol: 2004, 96(6);1245-55
[PubMed:15139916] [WorldCat.org] [DOI] (P p)

Barbara Setlow, Arturo Cabrera-Hernandez, Rosa Martha Cabrera-Martinez, Peter Setlow
Identification of aryl-phospho-beta-D-glucosidases in Bacillus subtilis.
Arch Microbiol: 2004, 181(1);60-7
[PubMed:14652714] [WorldCat.org] [DOI] (P p)