AroF

• Description: chorismate synthase
  
  

Gene name	aroF
Synonyms
Essential	no
Product	chorismate synthase
Function	biosynthesis of aromatic amino acids
Metabolic function and regulation of this protein in SubtiPathways: Phe, Tyr, Trp
MW, pI	40 kDa, 6.578
Gene length, protein length	1107 bp, 369 aa
Immediate neighbours	aroB, cheR
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU22710

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate (according to Swiss-Prot)

• Protein family: acetokinase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cytoplasm (according to Swiss-Prot)

Database entries

• Structure: 2O11 (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)

• UniProt: P31104

• KEGG entry: [2]

• E.C. number: 4.2.3.5

Expression and regulation

• Operon:
  • aroF-aroB-aroH (according to DBTBS)
  • aroF-aroB-aroH-trpE-trpD-trpC-trpF-trpB-trpA-hisC-tyrA-aroE (according to DBTBS)

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

S O Hoch
Mapping of the 5-methyltryptophan resistance locus in Bacillus subtilis.
J Bacteriol: 1974, 117(1);315-7
[PubMed:4202998] [WorldCat.org] [DOI] (P p)