PGP1087

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File:PGP1087.png
pGP1087: click to enlarge

General comments

Integrative plasmid for the fusion of 3x FLAG tag to the C-terminus of a protein, keeping the control of the expression under its natural promotor

  • E.coli: lowcopy plasmid, select for ampicillin
  • B. subtilis: no ori, select for erythromycin/lincomycin

Hints for the usage

  • amplify the last 300bp of your gene of interest,
  • skip the Stop-Codon of the gene in the revers primer
  • take care that the integration is in-frame

Antibody

  • dilute the anitbody 1:10000 (you can freeze the Blotto solution and reuse it)
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