GuaD
- Description: guanine deaminase, general stress protein
Gene name | guaD |
Synonyms | gde, yknA |
Essential | no |
Product | guanine deaminase |
Function | deamination of guanine to xanthine, purine salvage and interconversion |
Metabolic function and regulation of this protein in SubtiPathways: Purine salvage, Purine catabolism, Nucleotides (regulation) | |
MW, pI | 17 kDa, 4.999 |
Gene length, protein length | 468 bp, 156 aa |
Immediate neighbours | ohrB, metE |
Get the DNA and protein sequences (Barbe et al., 2009) | |
Genetic context This image was kindly provided by SubtiList
|
Contents
The gene
Basic information
- Locus tag: BSU13170
Phenotypes of a mutant
Database entries
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: Guanine + H2O = xanthine + NH3 (according to Swiss-Prot)
- Protein family: cytidine and deoxycytidylate deaminase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Interactions:
- Localization:
Database entries
- Structure: 1WKQ
- UniProt: O34598
- KEGG entry: [3]
- E.C. number: 3.5.4.3
Additional information
Expression and regulation
- Operon:
- Additional information:
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Dirk Höper, Uwe Völker, Michael Hecker
Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.
J Bacteriol: 2005, 187(8);2810-26
[PubMed:15805528]
[WorldCat.org]
[DOI]
(P p)
Shwu-Huey Liaw, Yu-Jui Chang, Cheng-Tsung Lai, Hui-Chuan Chang, Gu-Gang Chang
Crystal structure of Bacillus subtilis guanine deaminase: the first domain-swapped structure in the cytidine deaminase superfamily.
J Biol Chem: 2004, 279(34);35479-85
[PubMed:15180998]
[WorldCat.org]
[DOI]
(P p)
Lars Beier, Per Nygaard, Hanne Jarmer, Hans H Saxild
Transcription analysis of the Bacillus subtilis PucR regulon and identification of a cis-acting sequence required for PucR-regulated expression of genes involved in purine catabolism.
J Bacteriol: 2002, 184(12);3232-41
[PubMed:12029039]
[WorldCat.org]
[DOI]
(P p)
Per Nygaard, Søren M Bested, Kasper A K Andersen, Hans H Saxild
Bacillus subtilis guanine deaminase is encoded by the yknA gene and is induced during growth with purines as the nitrogen source.
Microbiology (Reading): 2000, 146 Pt 12;3061-3069
[PubMed:11101664]
[WorldCat.org]
[DOI]
(P p)