ParB

• Description: chromosome positioning near the pole and transport through the polar septum / antagonist of Soj-dependent inhibition of sporulation initiation
  
  

Gene name	spo0J
Synonyms	spo0JB
Essential	no
Product	site-specific DNA-binding protein
Function	chromosome positioning before asymmetric septation, specifying its orientation, and imposing directionality on its subsequent transport through the septum; required for sporulation (antagonist of Soj-dependent inhibition of sporulation initiation)
MW, pI	32 kDa, 8.417
Gene length, protein length	846 bp, 282 aa
Immediate neighbours	yyaC, soj
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU40960

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: parB family (according to Swiss-Prot)

• Paralogous protein(s): Noc

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: P26497

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:
  • repressed under conditions that trigger sporulation (Spo0A) PubMed

• Regulatory mechanism:
  • Spo0A: transcription repression PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Nora L Sullivan, Kathleen A Marquis, David Z Rudner
Recruitment of SMC by ParB-parS organizes the origin region and promotes efficient chromosome segregation.
Cell: 2009, 137(4);697-707
[PubMed:19450517] [WorldCat.org] [DOI] (I p)

Stephan Gruber, Jeff Errington
Recruitment of condensin to replication origin regions by ParB/SpoOJ promotes chromosome segregation in B. subtilis.
Cell: 2009, 137(4);685-96
[PubMed:19450516] [WorldCat.org] [DOI] (I p)

Heath Murray, Jeff Errington
Dynamic control of the DNA replication initiation protein DnaA by Soj/ParA.
Cell: 2008, 135(1);74-84
[PubMed:18854156] [WorldCat.org] [DOI] (I p)

Heath Murray, Henrique Ferreira, Jeff Errington
The bacterial chromosome segregation protein Spo0J spreads along DNA from parS nucleation sites.
Mol Microbiol: 2006, 61(5);1352-61
[PubMed:16925562] [WorldCat.org] [DOI] (P p)

Gonçalo Real, Sabine Autret, Elizabeth J Harry, Jeffery Errington, Adriano O Henriques
Cell division protein DivIB influences the Spo0J/Soj system of chromosome segregation in Bacillus subtilis.
Mol Microbiol: 2005, 55(2);349-67
[PubMed:15659156] [WorldCat.org] [DOI] (P p)

Virginie Molle, Masaya Fujita, Shane T Jensen, Patrick Eichenberger, José E González-Pastor, Jun S Liu, Richard Losick
The Spo0A regulon of Bacillus subtilis.
Mol Microbiol: 2003, 50(5);1683-701
[PubMed:14651647] [WorldCat.org] [DOI] (P p)

Ling Juan Wu, Jeff Errington
RacA and the Soj-Spo0J system combine to effect polar chromosome segregation in sporulating Bacillus subtilis.
Mol Microbiol: 2003, 49(6);1463-75
[PubMed:12950914] [WorldCat.org] [DOI] (P p)

S Autret, R Nair, J Errington
Genetic analysis of the chromosome segregation protein Spo0J of Bacillus subtilis: evidence for separate domains involved in DNA binding and interactions with Soj protein.
Mol Microbiol: 2001, 41(3);743-55
[PubMed:11532141] [WorldCat.org] [DOI] (P p)

A A Teleman, P L Graumann, D C Lin, A D Grossman, R Losick
Chromosome arrangement within a bacterium.
Curr Biol: 1998, 8(20);1102-9
[PubMed:9778525] [WorldCat.org] [DOI] (P p)

M A Cervin, G B Spiegelman, B Raether, K Ohlsen, M Perego, J A Hoch
A negative regulator linking chromosome segregation to developmental transcription in Bacillus subtilis.
Mol Microbiol: 1998, 29(1);85-95
[PubMed:9701805] [WorldCat.org] [DOI] (P p)

C D Webb, P L Graumann, J A Kahana, A A Teleman, P A Silver, R Losick
Use of time-lapse microscopy to visualize rapid movement of the replication origin region of the chromosome during the cell cycle in Bacillus subtilis.
Mol Microbiol: 1998, 28(5);883-92
[PubMed:9663676] [WorldCat.org] [DOI] (P p)

P J Lewis, J Errington
Direct evidence for active segregation of oriC regions of the Bacillus subtilis chromosome and co-localization with the SpoOJ partitioning protein.
Mol Microbiol: 1997, 25(5);945-54
[PubMed:9364919] [WorldCat.org] [DOI] (P p)

P Glaser, M E Sharpe, B Raether, M Perego, K Ohlsen, J Errington
Dynamic, mitotic-like behavior of a bacterial protein required for accurate chromosome partitioning.
Genes Dev: 1997, 11(9);1160-8
[PubMed:9159397] [WorldCat.org] [DOI] (P p)

M E Sharpe, J Errington
The Bacillus subtilis soj-spo0J locus is required for a centromere-like function involved in prespore chromosome partitioning.
Mol Microbiol: 1996, 21(3);501-9
[PubMed:8866474] [WorldCat.org] [DOI] (P p)

T H Mysliwiec, J Errington, A B Vaidya, M G Bramucci
The Bacillus subtilis spo0J gene: evidence for involvement in catabolite repression of sporulation.
J Bacteriol: 1991, 173(6);1911-9
[PubMed:1900505] [WorldCat.org] [DOI] (P p)