PhoR

• Description: two-component sensor kinase, regulation of phosphate metabolism
  
  

Gene name	phoR
Synonyms
Essential	no
Product	two-component sensor kinase (OmpR family)
Function	regulation of phosphate metabolism
MW, pI	64 kDa, 5.957
Gene length, protein length	1737 bp, 579 aa
Immediate neighbours	polA, phoP
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU29100

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + protein L-histidine = ADP + protein N-phospho-L-histidine (according to Swiss-Prot)

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: two transmembrane segments, C-terminal histidine phosphotransferase domain

• Cofactor(s):

• Effectors of protein activity:

• Interactions: PhoP-PhoR

• Localization:

Database entries

• Structure: 3CWF

• Swiss prot entry: P23545

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: phoP-phoR

• Sigma factor: SigA

• Regulation: induced by phosphate limitation (PhoP), carbon catabolite repression (CcpA)
  • expressed under conditions of phosphate limitation (PhoP) PubMed

• Regulatory mechanism: PhoP: transcription activation, CcpA: transcription repression
  • PhoP: transcription activation PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Marion Hulett, University of Illinois at Chicago, USA Homepage

References

Salbi Paul, Stephanie Birkey, Wei Liu, F Marion Hulett
Autoinduction of Bacillus subtilis phoPR operon transcription results from enhanced transcription from EsigmaA- and EsigmaE-responsive promoters by phosphorylated PhoP.
J Bacteriol: 2004, 186(13);4262-75
[PubMed:15205429] [WorldCat.org] [DOI] (P p)

Zoltán Prágai, Nicholas E E Allenby, Nicola O'Connor, Sarah Dubrac, Georges Rapoport, Tarek Msadek, Colin R Harwood
Transcriptional regulation of the phoPR operon in Bacillus subtilis.
J Bacteriol: 2004, 186(4);1182-90
[PubMed:14762014] [WorldCat.org] [DOI] (P p)

1. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed