LevF

• Description: fructose-specific phosphotransferase system, EIIC component
  
  

Gene name	levF
Synonyms	sacL
Essential	no
Product	fructose-specific phosphotransferase system, EIIC component
Function	fructose uptake and phosphorylation
Metabolic function and regulation of this protein in SubtiPathways: Sugar catabolism
MW, pI	27 kDa, 4.395
Gene length, protein length	807 bp, 269 aa
Immediate neighbours	levG, levE
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27050

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: PTS permease, mannose permease (Man) family PubMed

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions: LevF-LevG

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• Structure:

• Swiss prot entry: P26381

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon: levD-levE-levF-levG-sacC

• Sigma factor: SigL

• Regulation: repressed by glucose (CcpA) , carbon catabolite repression, induction by fructose

• Regulatory mechanism: CcpA: transcription repression, catabolite repression: transcription repression by CcpA, transcription activator LevR is less active in the presence of glucose; induction: transcription activation by LevR

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Reizer et al. (1999) Novel phosphotransferase system genes revealed by genome analysis - the complete complement of PTS proteins encoded within the genome of Bacillus subtilis. Microbiology 145: 3419-3429 PubMed

1. Martin-Verstraete, I., Débarbouillé, M., Klier, A., and Rapoport, G. (1990) Levanase operon of Bacillus subtilis includes a fructose-specific phosphotransferase system regulating the expression of the operon. J Mol Biol 214: 657-671. PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed