Sandbox

• Description: aspartyl-tRNA synthetase
  
  

Gene name	aspS
Synonyms
Essential	yes PubMed
Product	aspartyl-tRNA synthetase
Function	translation
MW, pI	65 kDa, 4.654
Gene length, protein length	1776 bp, 592 aa
Immediate neighbours	bsrA, hisS
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU27550

Phenotypes of a mutant

essential PubMed

Database entries

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp) (according to Swiss-Prot)

• Protein family: class-II aminoacyl-tRNA synthetase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylated on ser/ thr/ tyr PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• Swiss prot entry: O32038

• KEGG entry: [2]

• E.C. number: 6.1.1.12

Expression and regulation

• Operon:

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Lévine et al. (2006) Analysis of the dynamic Bacillus subtilis Ser/Thr/Tyr phosphoproteome implicated in a wide variety of cellular processes. Proteomics 6: 2157-2173 PubMed
2. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed