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• Description: general stress protein, survival of ethanol stress, spore coat protein
  
  

Gene name	yaaH
Synonyms
Essential	no
Product	spore coat protein
Function	survival of ethanol stress
MW, pI	48 kDa, 5.618
Gene length, protein length	1281 bp, 427 aa
Immediate neighbours	dgk, yaaI
Get the DNA and protein sequences (Barbe et al., 2009)
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Locus tag: BSU00160

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: spore wall (according to Swiss-Prot), spore coat PubMed

Database entries

• Structure:

• Swiss prot entry: P37531

• KEGG entry: [3]

• E.C. number:

Additional information

required for survival of ethanol stress PubMed

Expression and regulation

• Operon: yaaH PubMed

• Sigma factor: SigE PubMed, SigB PubMed

• Regulation: induced by stress (SigB) PubMed, expressed during sporulation PubMed

• Regulatory mechanism:

• Additional information: the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

1. Höper et al. (2005) Comprehensive Characterization of the Contribution of Individual SigB-Dependent General Stress Genes to Stress Resistance of Bacillus subtilis. J. Bact. 187: 2810-2826 PubMed
2. Kodama et al. (1999) The Bacillus subtilis yaaH gene is transcribed by SigE RNA polymerase during sporulation, and its product is involved in germination of spores.J. Bacteriol. 181: 4584-4591. PubMed
3. Höper et al. (2005) Comprehensive characterization of the contribution of individual SigB-dependent general stress genes to stress resistance of Bacillus subtilis.J.Bacteriol. 187: 2810-2826. PubMed
4. Aronson (2002) Sporulation and delta-endotoxin synthesis by Bacillus thuringiensis.Cell. Mol. Life Sci. 59: 417-425. PubMed