AcoA

• Description: acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
  
  

Gene name	acoA
Synonyms	yfjK
Essential	no
Product	acetoin dehydrogenase E1 component (TPP-dependent alpha subunit)
Function	acetoin utilization
MW, pI	35 kDa, 4.912
Gene length, protein length	999 bp, 333 aa
Immediate neighbours	yfjL, acoB
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

The gene

Basic information

• Coordinates:

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: Cytoplasm (Homogeneous) PubMed

Database entries

• Structure:

• Swiss prot entry:

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: acoA-acoB-acoC-acoL PubMed

• Sigma factor: SigL PubMed

• Regulation: repressed by glucose (CcpA) , induced by acetoin (AcoR) PubMed

• Regulatory mechanism: AcoR: transcription activation (interaction with SigL-containing RNA polymerase) PubMed

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

Labs working on this gene/protein

Michel Debarbouille, Pasteur Institute, Paris, France Homepage

References

1. Blencke et al. (2003) Transcriptional profiling of gene expression in response to glucose in Bacillus subtilis: regulation of the central metabolic pathways. Metab Eng. 5: 133-149
2. Meile et al. (2006) Systematic localisation of proteins fused to the green fluorescent protein in Bacillus subtilis: identification of new proteins at the DNA replication factory Proteomics 6: 2135-2146. PubMed
3. Ali, N. O., Bignon, J., Rapoport, G., and Débarbouillé, M. (2001) Regulation of the acetoin catabolic pathway is controlled by sigma L in Bacillus subtilis. J. Bacteriol. 183, 2497-2504. PubMed
4. Author1, Author2 & Author3 (year) Title Journal volume: page-page. PubMed