PfeT

• Description: Fe(II) efflux pump, P1B4 -type ATPase, protects the cell against iron intoxication
  
  

Gene name	zosA
Synonyms	ykvW
Essential	no
Product	Fe(II) efflux pump
Function	protection against toxic iron
Gene expression levels in SubtiExpress: pfeT
Metabolic function and regulation of this protein in SubtiPathways: PfeT
MW, pI	68 kDa, 4.934
Gene length, protein length	1911 bp, 637 aa
Immediate neighbours	stoA, papB
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins

This gene is a member of the following regulons

PerR regulon

The gene

Basic information

• Locus tag: BSU13850

Phenotypes of a mutant

• • sensitivity to iron, this can be suppressed by low levels of Mn(II) PubMed
  • reduced genetic competence, can be rescued by the addition of excess zinc PubMed
  • reduced expression of comK, control is exerted at the post-transcriptional level and can be rescued by the addition of excess zinc PubMed

Database entries

• BsubCyc: BSU13850

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: ATP + H₂O + Zn²⁺(In) = ADP + phosphate + Zn²⁺(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase PubMed

• Protein family: Type IB subfamily (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization: cell membrane (according to Swiss-Prot)

Database entries

• BsubCyc: BSU13850

• Structure:

• UniProt: O31688

• KEGG entry: [2]

• E.C. number: 3.6.3.5

Expression and regulation

• Operon: pfeT PubMed

• Expression browser: pfeT PubMed

• Sigma factor: SigA PubMed

• Regulation: induced by hydrogen peroxide (PerR) PubMed

• Regulatory mechanism:
  • PerR: transcription repression PubMed

• Additional information:
  • A ncRNA is predicted between stoA and zosA PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Guohua Guan, Azul Pinochet-Barros, Ahmed Gaballa, Sarju J Patel, José M Argüello, John D Helmann
PfeT, a P1B4 -type ATPase, effluxes ferrous iron and protects Bacillus subtilis against iron intoxication.
Mol Microbiol: 2015, 98(4);787-803
[PubMed:26261021] [WorldCat.org] [DOI] (I p)

Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502] [WorldCat.org] [DOI] (I p)

Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796] [WorldCat.org] [DOI] (I p)

Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235] [WorldCat.org] [DOI] (P p)

John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338] [WorldCat.org] [DOI] (P p)

Ahmed Gaballa, John D Helmann
A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis.
Mol Microbiol: 2002, 45(4);997-1005
[PubMed:12180919] [WorldCat.org] [DOI] (P p)