PfeT
- Description: Fe(II) efflux pump, P1B4 -type ATPase, protects the cell against iron intoxication
Gene name | zosA |
Synonyms | ykvW |
Essential | no |
Product | Fe(II) efflux pump |
Function | protection against toxic iron |
Gene expression levels in SubtiExpress: pfeT | |
Metabolic function and regulation of this protein in SubtiPathways: PfeT | |
MW, pI | 68 kDa, 4.934 |
Gene length, protein length | 1911 bp, 637 aa |
Immediate neighbours | stoA, papB |
Sequences | Protein DNA DNA_with_flanks |
Genetic context This image was kindly provided by SubtiList
| |
Expression at a glance PubMed |
Contents
Categories containing this gene/protein
transporters/ other, trace metal homeostasis (Cu, Zn, Ni, Mn, Mo), membrane proteins
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU13850
Phenotypes of a mutant
Database entries
- BsubCyc: BSU13850
- DBTBS entry: no entry
- SubtiList entry: [1]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + H2O + Zn2+(In) = ADP + phosphate + Zn2+(Out) (according to Swiss-Prot) P-type zinc-transporting ATPase PubMed
- Protein family: Type IB subfamily (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification:
- Cofactor(s):
- Effectors of protein activity:
- Localization: cell membrane (according to Swiss-Prot)
Database entries
- BsubCyc: BSU13850
- Structure:
- UniProt: O31688
- KEGG entry: [2]
- E.C. number: 3.6.3.5
Additional information
Expression and regulation
- Operon: pfeT PubMed
Biological materials
- Mutant:
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
Guohua Guan, Azul Pinochet-Barros, Ahmed Gaballa, Sarju J Patel, José M Argüello, John D Helmann
PfeT, a P1B4 -type ATPase, effluxes ferrous iron and protects Bacillus subtilis against iron intoxication.
Mol Microbiol: 2015, 98(4);787-803
[PubMed:26261021]
[WorldCat.org]
[DOI]
(I p)
Mitsuo Ogura
ZnuABC and ZosA zinc transporters are differently involved in competence development in Bacillus subtilis.
J Biochem: 2011, 150(6);615-25
[PubMed:21813502]
[WorldCat.org]
[DOI]
(I p)
Irnov Irnov, Cynthia M Sharma, Jörg Vogel, Wade C Winkler
Identification of regulatory RNAs in Bacillus subtilis.
Nucleic Acids Res: 2010, 38(19);6637-51
[PubMed:20525796]
[WorldCat.org]
[DOI]
(I p)
Mayuree Fuangthong, John D Helmann
Recognition of DNA by three ferric uptake regulator (Fur) homologs in Bacillus subtilis.
J Bacteriol: 2003, 185(21);6348-57
[PubMed:14563870]
[WorldCat.org]
[DOI]
(P p)
Ahmed Gaballa, John D Helmann
Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co and Cu efflux systems.
Biometals: 2003, 16(4);497-505
[PubMed:12779235]
[WorldCat.org]
[DOI]
(P p)
John D Helmann, Ming Fang Winston Wu, Ahmed Gaballa, Phil A Kobel, Maud M Morshedi, Paul Fawcett, Chris Paddon
The global transcriptional response of Bacillus subtilis to peroxide stress is coordinated by three transcription factors.
J Bacteriol: 2003, 185(1);243-53
[PubMed:12486061]
[WorldCat.org]
[DOI]
(P p)
Ahmed Gaballa, Tao Wang, Rick W Ye, John D Helmann
Functional analysis of the Bacillus subtilis Zur regulon.
J Bacteriol: 2002, 184(23);6508-14
[PubMed:12426338]
[WorldCat.org]
[DOI]
(P p)
Ahmed Gaballa, John D Helmann
A peroxide-induced zinc uptake system plays an important role in protection against oxidative stress in Bacillus subtilis.
Mol Microbiol: 2002, 45(4);997-1005
[PubMed:12180919]
[WorldCat.org]
[DOI]
(P p)