HemQ

• Description: heme-binding protein, essential for heme biosynthesis
  
  

Gene name	hemQ
Synonyms	ipa-87r, ywfI
Essential	no
Product	heme-binding protein
Function	biosynthesis of heme
Gene expression levels in SubtiExpress: hemQ
Metabolic function and regulation of this protein in SubtiPathways: HemQ
MW, pI	29 kDa, 4.972
Gene length, protein length	762 bp, 254 aa
Immediate neighbours	pta, bacG
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis of cofactors, phosphoproteins

The gene

Basic information

• Locus tag: BSU37670

Phenotypes of a mutant

defect in heme biosynthesis

Database entries

• BsubCyc: BSU37670

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family: UPF0447 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification: phosphorylation on Ser-41 PubMed

• Cofactor(s): heme

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU37670

• Structure: 1T0T (Geobacillus stearothermophilus)

• UniProt: P39645

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: hemQ PubMed

• Expression browser: hemQ PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 358 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 1041 PubMed

Biological materials

• Mutant:

• Expression vector:
  • pGP2643: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
  • pGP2644: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab

• lacZ fusion:

• GFP fusion:

• two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

• FLAG-tag vector:
  • pGP2658 hemQ-3xFLAG ermC (based on pBQ200), available in Jörg Stülke's lab

• Antibody:

References

Tamara A Dailey, Tye O Boynton, Angela-Nadia Albetel, Svetlana Gerdes, Michael K Johnson, Harry A Dailey
Discovery and Characterization of HemQ: an essential heme biosynthetic pathway component.
J Biol Chem: 2010, 285(34);25978-86
[PubMed:20543190] [WorldCat.org] [DOI] (I p)

Boris Macek, Ivan Mijakovic, Jesper V Olsen, Florian Gnad, Chanchal Kumar, Peter R Jensen, Matthias Mann
The serine/threonine/tyrosine phosphoproteome of the model bacterium Bacillus subtilis.
Mol Cell Proteomics: 2007, 6(4);697-707
[PubMed:17218307] [WorldCat.org] [DOI] (P p)

E Presecan, I Moszer, L Boursier, H Cruz Ramos, V de la Fuente, M-F Hullo, C Lelong, S Schleich, A Sekowska, B H Song, G Villani, F Kunst, A Danchin, P Glaser
The Bacillus subtilis genome from gerBC (311 degrees) to licR (334 degrees).
Microbiology (Reading): 1997, 143 ( Pt 10);3313-3328
[PubMed:9353933] [WorldCat.org] [DOI] (P p)