BrnQ

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Revision as of 14:50, 4 February 2015 by Jstuelk (talk | contribs) (Extended information on the protein)
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Gene name brnQ
Synonyms yrdJ
Essential no
Product branched-chain amino acid transporter
Function uptake of branched-chain amino acids
Gene expression levels in SubtiExpress: brnQ
Metabolic function and regulation of this protein in SubtiPathways:
brnQ
MW, pI 46 kDa, 9.777
Gene length, protein length 1320 bp, 440 aa
Immediate neighbours yrdK, azlD
Sequences Protein DNA DNA_with_flanks
Genetic context
BrnQ context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
BrnQ expression.png















Categories containing this gene/protein

transporters/ other, biosynthesis/ acquisition of amino acids

This gene is a member of the following regulons

AzlB regulon

The gene

Basic information

  • Locus tag: BSU26690

Phenotypes of a mutant

  • no phenotype for the single mutant, the triple bcaP brnQ braB mutant is strongly impaired in the transport of isoleucine and valine at low concentrations PubMed

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • high affinity uptake of isoleucine and valine PubMed
  • Protein family: branched chain amino acid transporter family (according to Swiss-Prot)
  • Paralogous protein(s): BraB

Extended information on the protein

  • Kinetic information:
    • for Ile: Km ≈ 16 μM and Vmax ≈ 25 nmol/min/ mg protein at 37°C PubMed
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulatory mechanism: AzlB: transcriptional repression PubMed
  • Additional information:

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References