Scr

• Description: 4.5 S RNA
  
  

Gene name	scr
Synonyms
Essential	yes
Product	small cytoplasmic RNA (scRNA, 4.5S RNA), signal recognition particle-like (SRP) component
Function	presecretory protein translocation
Interactions involving this RNA in SubtInteract: Scr
Immediate neighbours	yaaJ, dnaX
Gene sequence (+200bp)	Protein sequence
Genetic context This image was kindly provided by SubtiList

Categories containing this gene/protein

protein secretion, essential genes, ncRNA

The gene

Basic information

• Locus tag:

Phenotypes of a mutant

• the gene is essential PubMed

Database entries

• BsubCyc: BSU_misc_RNA_2

• DBTBS entry: no entry

• SubtiList entry: [1]

• Structure:

The RNA

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

Extended information on the RNA

• Kinetic information

• Interactions:
  • Scr-Ffh in the signal recognition particle

• Localization

Database entries

• BsubCyc: BSU_misc_RNA_2

• Structure: 4WFL PubMed

Expression and regulation

• Operon: yaaJ-scr, scr

• Sigma factor: SigA (both promoters)

• Regulation:

• Regulatory mechanism:

• Additional information: transcribed as 354 nt precursor, cleaved at the 5' end by RNase III and at the 3' site by RNase J1 to yield a 275 nt molecule, final trimming to 271 nt sc RNA by RNase PH PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

Labs working on this gene/protein

David Bechhofer, Mount Sinai School, New York, USA Homepage

References

Reviews

Zhen Kang, Sen Yang, Guocheng Du, Jian Chen
Molecular engineering of secretory machinery components for high-level secretion of proteins in Bacillus species.
J Ind Microbiol Biotechnol: 2014, 41(11);1599-607
[PubMed:25212246] [WorldCat.org] [DOI] (I p)

Original publications

Georg Kempf, Klemens Wild, Irmgard Sinning
Structure of the complete bacterial SRP Alu domain.
Nucleic Acids Res: 2014, 42(19);12284-94
[PubMed:25270875] [WorldCat.org] [DOI] (I p)

Shiyi Yao, Joshua B Blaustein, David H Bechhofer
Processing of Bacillus subtilis small cytoplasmic RNA: evidence for an additional endonuclease cleavage site.
Nucleic Acids Res: 2007, 35(13);4464-73
[PubMed:17576666] [WorldCat.org] [DOI] (I p)

K Nakamura, S Yahagi, T Yamazaki, K Yamane
Bacillus subtilis histone-like protein, HBsu, is an integral component of a SRP-like particle that can bind the Alu domain of small cytoplasmic RNA.
J Biol Chem: 1999, 274(19);13569-76
[PubMed:10224127] [WorldCat.org] [DOI] (P p)

T Yamazaki, S Yahagi, K Nakamura, K Yamane
Depletion of Bacillus subtilis histone-like protein, HBsu, causes defective protein translocation and induces upregulation of small cytoplasmic RNA.
Biochem Biophys Res Commun: 1999, 258(1);211-4
[PubMed:10222262] [WorldCat.org] [DOI] (P p)

A Oguro, H Kakeshita, K Nakamura, K Yamane, W Wang, D H Bechhofer
Bacillus subtilis RNase III cleaves both 5'- and 3'-sites of the small cytoplasmic RNA precursor.
J Biol Chem: 1998, 273(31);19542-7
[PubMed:9677377] [WorldCat.org] [DOI] (P p)

H Takamatsu, K Bunai, T Horinaka, A Oguro, K Nakamura, K Watabe, K Yamane
Identification of a region required for binding to presecretory protein in Bacillus subtilis Ffh, a homologue of the 54-kDa subunit of mammalian signal recognition particle.
Eur J Biochem: 1997, 248(2);575-82
[PubMed:9346318] [WorldCat.org] [DOI] (P p)

K Kurita, K Honda, S Suzuma, H Takamatsu, K Nakamura, K Yamane
Identification of a region of Bacillus subtilis Ffh, a homologue of mammalian SRP54 protein, that is essential for binding to small cytoplasmic RNA.
J Biol Chem: 1996, 271(22);13140-6
[PubMed:8662730] [WorldCat.org] [DOI] (P p)

T Shibata, Y Nakamura, K Nakamura, K Yamane
Depletion of small cytoplasmic RNA confers fusidic-acid resistance on Bacillus subtilis.
Biochem Biophys Res Commun: 1995, 210(2);317-23
[PubMed:7538759] [WorldCat.org] [DOI] (P p)

M Nishiguchi, K Honda, R Amikura, K Nakamura, K Yamane
Structural requirements of Bacillus subtilis small cytoplasmic RNA for cell growth, sporulation, and extracellular enzyme production.
J Bacteriol: 1994, 176(1);157-65
[PubMed:7506707] [WorldCat.org] [DOI] (P p)

K Nakamura, M Minemura, M Nishiguchi, K Honda, A Nakamura, K Yamane
Conserved residues and secondary structure found in small cytoplasmic RNAs from thirteen Bacillus species.
Nucleic Acids Res: 1992, 20(19);5227-8
[PubMed:1383945] [WorldCat.org] [DOI] (P p)

K Nakamura, Y Imai, A Nakamura, K Yamane
Small cytoplasmic RNA of Bacillus subtilis: functional relationship with human signal recognition particle 7S RNA and Escherichia coli 4.5S RNA.
J Bacteriol: 1992, 174(7);2185-92
[PubMed:1372600] [WorldCat.org] [DOI] (P p)

J C Struck, R K Hartmann, H Y Toschka, V A Erdmann
Transcription and processing of Bacillus subtilis small cytoplasmic RNA.
Mol Gen Genet: 1989, 215(3);478-82
[PubMed:2468993] [WorldCat.org] [DOI] (P p)