YodF

• Description: similar to amino acid permease
  
  

Gene name	yodF
Synonyms
Essential	no
Product	unknown
Function	unknown
Gene expression levels in SubtiExpress: yodF
Metabolic function and regulation of this protein in SubtiPathways: YodF
MW, pI	53 kDa, 9.682
Gene length, protein length	1488 bp, 496 aa
Immediate neighbours	yoyD, ctpA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

transporters/ other, putative amino acid transporter, membrane proteins

This gene is a member of the following regulons

TnrA regulon

The gene

Basic information

• Locus tag: BSU19580

Database entries

• BsubCyc: BSU19580

• DBTBS entry: no entry

• SubtiList entry: [1]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:
  • in some database, the protein is annotated as being similar to proline permease, however, it is not involved in proline transport PubMed

• Protein family: solute:sodium symporter family (SSS family)

• Paralogous protein(s): YhjB

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:
  • membrane PubMed

Database entries

• BsubCyc: BSU19580

• Structure:

• UniProt: O34745

• KEGG entry: [2]

• E.C. number:

Expression and regulation

• Operon:

• Expression browser: yodF PubMed

• Sigma factor:

• Regulation: repressed by casamino acids PubMed
  • repressed in the absence of good nitrogen sources (glutamine or ammonium) (TnrA) PubMed

• Regulatory mechanism:
  • TnrA: transcription repression PubMed

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium): 445 PubMed
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 807 PubMed

Biological materials

• Mutant:
  • available in Erhard Bremer's lab PubMed
  • GP1887 (kan) available in Jörg Stülke's lab
  • GP1889 (ytnA replaced by a spec cassette and yodF replaced by a kan cassette) available in Jörg Stülke's lab

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Adrienne Zaprasis, Tamara Hoffmann, Lorena Stannek, Katrin Gunka, Fabian M Commichau, Erhard Bremer
The γ-aminobutyrate permease GabP serves as the third proline transporter of Bacillus subtilis.
J Bacteriol: 2014, 196(3);515-26
[PubMed:24142252] [WorldCat.org] [DOI] (I p)

Hannes Hahne, Susanne Wolff, Michael Hecker, Dörte Becher
From complementarity to comprehensiveness--targeting the membrane proteome of growing Bacillus subtilis by divergent approaches.
Proteomics: 2008, 8(19);4123-36
[PubMed:18763711] [WorldCat.org] [DOI] (I p)

Ken-ichi Yoshida, Hirotake Yamaguchi, Masaki Kinehara, Yo-hei Ohki, Yoshiko Nakaura, Yasutaro Fujita
Identification of additional TnrA-regulated genes of Bacillus subtilis associated with a TnrA box.
Mol Microbiol: 2003, 49(1);157-65
[PubMed:12823818] [WorldCat.org] [DOI] (P p)

Ulrike Mäder, Georg Homuth, Christian Scharf, Knut Büttner, Rüdiger Bode, Michael Hecker
Transcriptome and proteome analysis of Bacillus subtilis gene expression modulated by amino acid availability.
J Bacteriol: 2002, 184(15);4288-95
[PubMed:12107147] [WorldCat.org] [DOI] (P p)