LicH

• Description: 6-phospho-beta-glucosidase
  
  

Gene name	licH
Synonyms	celD, celF
Essential	no
Product	6-phospho-beta-glucosidase
Function	lichenan utilization
Gene expression levels in SubtiExpress: licH
Metabolic function and regulation of this protein in SubtiPathways: licH
MW, pI	48 kDa, 5.183
Gene length, protein length	1326 bp, 442 aa
Immediate neighbours	ywaA, licA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

utilization of specific carbon sources, sporulation proteins

This gene is a member of the following regulons

CcpA regulon, LicR regulon

The gene

Basic information

• Locus tag: BSU38560

Database entries

• BsubCyc: BSU38560

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: 6-phospho-beta-D-glucosyl-(1,4)-D-glucose + H₂O = D-glucose + D-glucose 6-phosphate (according to Swiss-Prot)

• Protein family: glycosyl hydrolase 4 family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU38560

• Structure: 1S6Y (Geobacillus stearothermophilus)

• UniProt: P46320

• KEGG entry: [3]

• E.C. number: 3.2.1.86

Expression and regulation

• Operon: licB-licC-licA-licH PubMed

• Expression browser: licH PubMed

• Sigma factor: SigA PubMed

• Regulation:
  • carbon catabolite repression (CcpA) PubMed
  • induction by oligomeric ß-glucosides (LicR)PubMed

• Regulatory mechanism:
  • CcpA: transcription repression PubMed
  • LicR: transcription activation PubMed

• Additional information:
  • number of protein molecules per cell (complex medium with amino acids, without glucose): 363 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody:

References

Takashi Inaoka, Takenori Satomura, Yasutaro Fujita, Kozo Ochi
Novel gene regulation mediated by overproduction of secondary metabolite neotrehalosadiamine in Bacillus subtilis.
FEMS Microbiol Lett: 2009, 291(2);151-6
[PubMed:19087206] [WorldCat.org] [DOI] (I p)

Le Thi Tam, Christine Eymann, Dirk Albrecht, Rabea Sietmann, Frieder Schauer, Michael Hecker, Haike Antelmann
Differential gene expression in response to phenol and catechol reveals different metabolic activities for the degradation of aromatic compounds in Bacillus subtilis.
Environ Microbiol: 2006, 8(8);1408-27
[PubMed:16872404] [WorldCat.org] [DOI] (P p)

S Tobisch, J Stülke, M Hecker
Regulation of the lic operon of Bacillus subtilis and characterization of potential phosphorylation sites of the LicR regulator protein by site-directed mutagenesis.
J Bacteriol: 1999, 181(16);4995-5003
[PubMed:10438772] [WorldCat.org] [DOI] (P p)

S Tobisch, P Glaser, S Krüger, M Hecker
Identification and characterization of a new beta-glucoside utilization system in Bacillus subtilis.
J Bacteriol: 1997, 179(2);496-506
[PubMed:8990303] [WorldCat.org] [DOI] (P p)