Apt

• Description: adenine phosphoribosyltransferase, universally conserved protein
  
  

Gene name	apt
Synonyms
Essential	no
Product	adenine phosphoribosyltransferase
Function	purine salvage and interconversion
Gene expression levels in SubtiExpress: apt
Metabolic function and regulation of this protein in SubtiPathways: apt
MW, pI	18 kDa, 4.701
Gene length, protein length	510 bp, 170 aa
Immediate neighbours	relA, recJ
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

biosynthesis/ acquisition of nucleotides, universally conserved proteins, phosphoproteins

The gene

Basic information

• Locus tag: BSU27610

Database entries

• BsubCyc: BSU27610

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity: AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate (according to Swiss-Prot)

• Protein family: purine/pyrimidine phosphoribosyltransferase family (according to Swiss-Prot)

• Paralogous protein(s):

Extended information on the protein

• Kinetic information:

• Domains:

• Modification:
  • phosphorylated on Arg-85 PubMed

• Cofactor(s):

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• BsubCyc: BSU27610

• Structure: 2DY0 (from Escherichia coli k12, 56% identity, 66% similarity)

• UniProt: O34443

• KEGG entry: [3]

• E.C. number: 2.4.2.7

Additional information

universally conserved protein

Expression and regulation

• Operon: yrvE-apt PubMed

• Expression browser: apt PubMed

• Sigma factor:

• Regulation:

• Regulatory mechanism:

• Additional information:
  • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 1738 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 1189 PubMed
  • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 1706 PubMed

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: